EdRox requires an interdisciplinary effort from groups in biochemistry, biology and physics.

LIC (NL): Leiden University, Leiden Institute of Chemistry

Metalloproteins group
Scientist in charge: Prof. Dr. G.W. Canters
The MetProt group will provide theoretical and practical training in molecular biology, biological spectroscopy, protein engineering and surface chemistry. It has a vigorous research program in protein engineering, cellular metal homeostasis, protein-protein interactions, DNA repair, advanced protein spectroscopy and bionanotechnology.

LION (NL): Leiden University, Leiden Institute of Physics

Department of Biophysics
Scientist in charge: Prof. Dr. T.J. Aartsma
The group will provide state-of-the-art technology and expertise on fluorescence detection and analysis to the network, which is one of the keyelements of the Fluorox principle. The group has a strong and expanding research program in single-molecule biophysics specifically aimed at biomolecular systems.

TOR (IT): University of Torino, Department of Biology

Department of Biological Chemistry
Scientist in charge: Prof. Dr. G. Gilardi
Using a molecular Lego approach human P450 enzymes will be linked at DNA level to an electron transfer module (flavodoxin (FLD) or the P450 reductase domain (BM3) for communication with the electrode. Cysteine mutants will be engineered to assist linkage to the modified electrode surface.

OXF (UK): Oxford University, Department of Chemistry.

Central Research Laboratory
Scientist in charge: Dr. J.J. Davis
Surface analysis techniques will be applied to bio-engineered electrodes for voltametric analytical sensing: electron transfer and tunneling, analysis of topography, methods of control of electrochemical and mechanical coupling, design and self-assembly of biomolecular receptors, dip-pen nanolithography.

MOD (IT): University of Modena, Department of Chemistry

Department of Chemistry
Scientist in charge:Prof. Dr. M. Sola
The group will contribute to the immobilization of enzymes on the electrodes by SAMs, peptides and spacers, to measure the activity of the engineered proteins and to characterize the redox and morphological features of the protein monolayers, supported by a rational design of protein mutants.

NWC (UK): Newcastle University

Biomedical Sciences, Medical School
Scientist in charge: Dr. C. Dennison
The group will prepare, isolate and purify (and perform the initial characterization) of various mutated forms of redox enzymes (primarily laccases and nitrite reductases) for FluoRox applications. Fluorescent labeling of enzymes and mechanistic characterization of FluoRox electrodes will also be performed.

HUJI (IL): Hebrew University of Jerusalem

Institute of Chemistry
Scientist in charge: Prof. Dr. I. Willner
The group's contribution comprises functionalization of surfaces with redox proteins, site-specific covalent anchoring of proteins to surfaces, alignment of proteins on surfaces by reconstitution methods, and the structural characterization of biomolecule-modified surfaces by SPR, AFM, QCM.

UNL (PT): Universidade Nova de Lisboa

Instituto de Tecnologia Química e Biológica
Scientist in charge: Dr. Y. Astier
Instituto de Tecnologia Química e Biológica (ITQB) is a scientific research and advanced training institute of the Universidade Nova de Lisboa. Our mission is to develop scientifically recognized research and to provide training-through-research in chemistry and the life sciences, considering all levels of complexity and its potential applications, so as to contribute to the understanding of life’s mechanisms and ultimately benefit the whole society.

BIOM (NL): Biomade Technology Foundation

Biomade Technology Foundation
Scientist in charge: Prof. Dr. G. Robillard
Biomade's contribution includes: production, isolation and modification hydrophobins for coating the electrode surfaces; the incorporation of small molecule redox mediators and the coupling of redox enzymes to these surfaces; characterization of enzyme stability and activity (enzymology, amperometry.

UoL (UK): University of Leeds

Institute of Membrane and Systems Biology
Scientist in charge: Dr. Lars J.C. Jeuken
Our intake in this project is to develop novel electrode materials that interact directly with membrane-embedded redox proteins either in a planar configuration or in the form of intact vesicles. These 'membrane-electrodes' are characterised with a broad spectrum of tools, including Atomic Force Spectroscopy and electrochemical tools