EdRox: Project description

Seven academic groups with established track records in education and complementary expertise in research participate in this intersectorial and interdisciplinary network together with two SMEs which are active in biotechnology.

Implementation

The implementation of the FluoRox principle into a working prototype FluoRox sensor requires four steps. The first step consists of the development of the element that actually performs the detection, i.e., the protein/enzyme. We have chosen three enzymes,

  • nitrite reductase (NiR, from Alcaligenes faecalis),
  • cytochrome P450 BM3 (P450, from Bacillus megatherium) and
  • laccase (LAC, from Streptomyces coelicolor, Bacillus subtilis and Trametes versicolor)

and their reaction partners for incorporation into a FluoRox sensor. These will lead to prototypes of sensors that stand for three major types of application: environmental monitoring (NiR), medical and pharmaceutical diagnostics (P450, NiR), industrial bulk and fine-chemical processes (LAC). The second step involves electrode preparation and immobilization of the enzyme/protein onto it. The third step is electrochemical characterization of the construct (electrode with immobilized protein/enzyme). The fourth and last step comprises construction and testing of a prototype FluoRox sensor. Expertise, strains and equipment are available in the consortium to secure batchwise production of purified enzymes at the 10-100 mg (NiR, LAC) or the 1-10 mg scale (P450).